DBCO-PEG4-Biotin is widely used in bioconjugation and labeling applications due to its unique properties. The DBCO (dibenzocyclooctyne) group enables efficient click chemistry reactions with azide-containing molecules, allowing for rapid and specific conjugation without the need for catalysts. The PEG4 (polyethylene glycol) spacer provides flexibility and solubility, enhancing the stability and accessibility of the biotin moiety. Biotin, a well-known ligand for avidin and streptavidin, facilitates strong and specific binding, making this compound ideal for applications such as protein labeling, affinity purification, and detection in assays like ELISA or Western blotting. Its versatility makes it a valuable tool in molecular biology, diagnostics, and drug development.