This compound is widely used in bioconjugation and drug delivery systems due to its reactive amine group and polyethylene glycol (PEG) spacer. The PEG chain enhances solubility and reduces immunogenicity, making it ideal for modifying peptides, proteins, and other biomolecules. The tert-butyl ester group provides a protective moiety that can be selectively deprotected to expose a carboxylic acid, enabling further functionalization. It is particularly valuable in the development of antibody-drug conjugates (ADCs), where it serves as a linker to connect cytotoxic drugs to antibodies, ensuring targeted delivery to cancer cells. Additionally, it is employed in the synthesis of hydrogels and biomaterials for tissue engineering, where its biocompatibility and tunable properties are advantageous.