NH2-PEG-NH2 is widely used in bioconjugation and drug delivery systems due to its bifunctional nature. It serves as a linker to attach bioactive molecules, such as proteins, peptides, or drugs, to other compounds or surfaces. This chemical is particularly valuable in creating PEGylated therapeutics, which enhance the solubility, stability, and circulation time of drugs in the body. It is also employed in the development of hydrogels and nanoparticles for controlled drug release.

Additionally, NH2-PEG-NH2 is utilized in surface modification to improve biocompatibility and reduce immunogenicity in medical devices and implants. Its applications extend to research in diagnostics, where it aids in the immobilization of biomolecules on sensor surfaces for improved detection sensitivity.