DBCO-PEG12-acid is widely utilized in bioconjugation and click chemistry applications due to its unique properties. The DBCO (dibenzocyclooctyne) group enables strain-promoted azide-alkyne cycloaddition (SPAAC), a reaction that occurs without the need for a catalyst, making it highly efficient for labeling biomolecules in biological systems. The PEG12 (polyethylene glycol) spacer provides solubility, reduces steric hindrance, and enhances biocompatibility, making it suitable for use in vivo. The terminal carboxylic acid group allows for further functionalization, enabling conjugation to amines or other reactive groups via standard coupling reactions. This compound is particularly valuable in drug delivery systems, where it facilitates the attachment of targeting ligands or therapeutic agents to nanoparticles or antibodies. It is also used in the development of diagnostic probes, where it helps in tagging biomolecules for imaging or detection purposes. Additionally, DBCO-PEG12-acid is employed in surface modification strategies, enabling the creation of functionalized materials for biosensors or biomaterials research. Its versatility and efficiency make it a critical tool in advancing biomedical and biotechnological applications.