This compound is widely used in bioconjugation and click chemistry applications. It serves as a linker for attaching biomolecules, such as proteins, peptides, and antibodies, to other molecules or surfaces. The NHS ester group reacts efficiently with primary amines, enabling covalent bonding to biomolecules, while the DBCO group allows for strain-promoted azide-alkyne cycloaddition (SPAAC) reactions, which are highly specific and occur under mild conditions. This makes it particularly useful in drug delivery systems, diagnostic assays, and the development of targeted therapeutics. Its PEG4 spacer enhances solubility and reduces steric hindrance, improving the efficiency of conjugation processes.