Used primarily in bioconjugation and click chemistry applications, this compound facilitates the attachment of biomolecules to various surfaces or other molecules. Its NHS ester group reacts efficiently with primary amines, enabling the formation of stable amide bonds, while the DBCO group allows for strain-promoted azide-alkyne cycloaddition, a key reaction in bioorthogonal chemistry. This dual functionality makes it highly valuable in labeling proteins, peptides, and other biomolecules for research in drug delivery, diagnostics, and cellular imaging. Its PEG4 spacer enhances solubility and reduces steric hindrance, improving the efficiency of conjugation processes.