Amino-PEG12-alcohol is widely used in bioconjugation and pharmaceutical formulation due to its long, hydrophilic polyethylene glycol (PEG) spacer. The terminal amine and hydroxyl groups allow for selective chemical modifications, making it ideal for linking biomolecules such as peptides, proteins, or drugs to targeting agents, surfaces, or nanoparticles. Its extended PEG chain helps improve solubility, reduce aggregation, and minimize immunogenicity of conjugated compounds.

It is commonly employed in the development of PEGylated therapeutics, where it enhances the circulation half-life of active molecules by reducing renal clearance and shielding from proteolytic enzymes. In drug delivery systems, Amino-PEG12-alcohol serves as a key building block in linker design for antibody-drug conjugates (ADCs) and prodrugs, enabling controlled release and improved bioavailability.

Additionally, it is used in surface functionalization of medical devices and biosensors, where the amine group anchors to surfaces while the PEG segment resists non-specific protein adsorption. Its biocompatibility and flexibility also make it suitable for use in molecular probes and diagnostic reagents.