This chemical is widely used in bioconjugation and labeling applications due to its unique structure and properties. It is particularly valuable in fluorescence imaging and cellular studies, where the Cy5 dye component provides strong near-infrared fluorescence, enabling deep tissue imaging with minimal background interference. The PEG4 spacers enhance solubility and reduce nonspecific binding, making it suitable for attaching to biomolecules like proteins, peptides, or antibodies without disrupting their function. The hydroxycyclohexyl group offers additional stability and can be further modified for specific targeting or conjugation purposes. Its applications span across drug delivery systems, molecular probes, and diagnostic tools, where precise labeling and tracking are essential.