Isoamyl nitrate is primarily used as a fuel additive in high-performance engines, particularly in diesel and racing applications. It acts as a cetane improver, enhancing the combustion efficiency of diesel fuel by reducing ignition delay. This results in smoother engine operation, reduced knocking, and improved overall performance. Additionally, it is employed in some industrial processes as a solvent or reagent due to its chemical properties. In niche applications, it is also utilized in the synthesis of organic compounds, particularly in the preparation of esters and other derivatives.