Used primarily as a reference standard in analytical laboratories for the detection and quantification of organophosphorus compounds, especially in environmental and forensic analysis. It helps calibrate instruments such as gas chromatographs and mass spectrometers to ensure accurate identification of pesticide metabolites and chemical warfare agent degradation products. Due to its reactivity, it is also employed in research settings to study phosphorylation reactions and enzyme inhibition mechanisms. Strict handling protocols are followed due to its toxic and potentially hazardous nature.