Used primarily in research as a mutagenic compound, this chemical is notably applied in the Ames test to evaluate the mutagenic potential of new substances. It serves as a positive control due to its strong ability to induce genetic mutations in bacterial strains. It is also studied for its role in understanding carcinogenesis and DNA adduct formation, particularly in relation to heterocyclic aromatic amines found in cooked foods. Its application extends to toxicological studies assessing environmental and dietary exposure risks.