Cholic acid is widely used in the pharmaceutical industry as a key ingredient in the synthesis of bile acid-based drugs. It plays a crucial role in treating liver and gallbladder disorders by aiding in the digestion and absorption of fats and fat-soluble vitamins. Additionally, it is utilized in the production of ursodeoxycholic acid, a medication for dissolving gallstones and managing primary biliary cholangitis. In research, cholic acid serves as a reference standard for studying bile acid metabolism and related diseases. It is also employed in the development of drug delivery systems, enhancing the solubility and bioavailability of poorly water-soluble drugs. Furthermore, cholic acid derivatives are explored for their potential in anti-inflammatory and anti-cancer therapies.