Retrorsine is primarily used in scientific research, particularly in studies related to liver injury and hepatotoxicity. It serves as a tool compound to induce liver damage in experimental models, helping researchers investigate mechanisms of hepatic fibrosis, cirrhosis, and liver regeneration. Due to its toxic effects on liver sinusoidal endothelial cells and hepatocytes, retrorsine is often used in rodent models to create a compromised liver environment, which is especially useful in studies involving liver cell transplantation and stem cell differentiation into hepatocytes. Its ability to irreversibly inhibit liver cell proliferation makes it valuable for creating a selective growth advantage for transplanted cells, enabling better engraftment and assessment of cell-based therapies. Retrorsine is not used in clinical medicine due to its high toxicity and carcinogenic potential.