Used as a stable isotope-labeled internal standard in mass spectrometry (MS) and liquid chromatography-mass spectrometry (LC-MS) methods for the accurate quantification of deoxynivalenol (DON) in food and feed samples. Its primary application is in mycotoxin testing laboratories to ensure precise detection and measurement of DON contamination in cereals, grains, and processed food products. The 13C15-labeled structure provides a distinct mass shift, allowing clear differentiation from the native toxin while following the same chemical behavior during extraction and analysis. This improves method accuracy, compensates for matrix effects, and enhances reproducibility in high-throughput monitoring programs for food safety and regulatory compliance.