This compound is primarily utilized in the field of medicinal chemistry and drug development. It functions as a potent inhibitor of dihydrofolate reductase (DHFR), an enzyme crucial for folate metabolism and essential for DNA and RNA synthesis in cells. Its application is significant in the design of therapeutic agents targeting diseases such as cancer, where inhibiting DHFR can disrupt the growth and proliferation of malignant cells. Additionally, it is studied for its potential in treating autoimmune disorders, inflammatory conditions, and certain infectious diseases, as it can modulate immune responses and interfere with key biochemical pathways in abnormal cell growth. Research is ongoing to explore its efficacy and safety in preclinical and clinical settings.