Used in biochemical and pharmaceutical research, particularly for studying protein structure and function. It can be incorporated into protein structures in place of natural phenylalanine, enabling tracking or modification of protein properties, such as folding, enzyme activity, or interactions with other molecules.

Additionally, employed in drug development for protein-targeted therapeutics, where it enhances stability, specificity, or reduces degradation of drug molecules. Valuable in biological imaging due to fluorine groups detectable by NMR or PET techniques.

Also used in the synthesis of pharmaceutical intermediates, especially fluorinated peptides and proteins for structure-activity relationship studies. Fluorine substitutions improve metabolic stability and influence conformation, aiding medicinal chemistry in designing bioactive molecules and probing enzyme mechanisms to enhance pharmacokinetic properties of peptide-based drugs.