Perfluorotributylamine is widely used in the electronics industry as a heat transfer fluid due to its excellent thermal stability and non-conductive properties. It is commonly applied in cooling systems for high-performance electronic components, such as servers and power electronics, where efficient heat dissipation is critical.

In the medical field, it serves as a blood substitute and oxygen carrier in certain experimental and clinical settings. Its ability to dissolve large amounts of oxygen makes it useful in situations where traditional blood transfusions are not feasible.

Additionally, it is utilized in the manufacturing of specialized lubricants and coatings, particularly in environments requiring chemical inertness and resistance to extreme temperatures. Its hydrophobic and oleophobic properties also make it suitable for use in surface treatments to repel water and oil.

In analytical chemistry, it acts as a reference standard in mass spectrometry and other techniques due to its stability and well-defined chemical properties.

It is also employed in semiconductor manufacturing for cleaning and contaminant removal processes.