Used extensively as a fluorescent dye to detect amyloid fibrils and aggregates in biological samples. Binds specifically to the beta-sheet structures characteristic of amyloid proteins, making it a key tool in research on neurodegenerative diseases like Alzheimer's and Parkinson's. Upon binding, it exhibits a significant increase in fluorescence intensity, allowing for sensitive detection and quantification in both in vitro assays and tissue staining. Also applied in high-throughput screening for compounds that inhibit amyloid formation. Used in microscopy and flow cytometry due to its strong and specific signal. Additionally employed in studies of protein misfolding and aggregation kinetics.