Widely used as a fluorescent probe in biological and biochemical research, this compound is particularly effective for labeling and tracking biomolecules in cellular environments. Its strong fluorescence and affinity for nucleic acids make it suitable for DNA and RNA detection in assays and imaging techniques. It is also employed in membrane studies due to its ability to interact with lipid bilayers, allowing visualization of membrane structures and dynamics. Additionally, it serves as a stain in microscopy, offering high contrast and photostability for prolonged observation. Its dual positive charges enhance water solubility and promote binding to negatively charged biological polymers, improving its utility in electrophoresis and gel-based applications.