Used as a fluorescent probe in biochemical and analytical applications, particularly for detecting enzyme activity such as β-glucuronidase and esterase in cellular systems. Its structure allows for selective activation in the presence of specific enzymes, making it valuable in imaging and diagnostic assays. Also employed in research settings for labeling biomolecules due to its strong fluorescence upon deprotection or enzymatic cleavage. Commonly applied in cell-based assays and high-throughput screening for drug discovery.