Used as a fluorescent probe in biochemical and analytical applications due to its strong emission properties upon binding to specific biomolecules. Exhibits high sensitivity to environmental polarity, making it suitable for studying membrane dynamics and protein conformational changes. Also employed in the development of chemosensors for detecting metal ions and reactive oxygen species in cellular systems. Its nitrobenzothiazole core enhances photostability and cell permeability, allowing for live-cell imaging with minimal toxicity.