Used as a fluorescent probe in biological imaging due to its strong emission properties in lipid environments. It selectively accumulates in cell membranes, making it useful for visualizing membrane dynamics and detecting lipid-rich domains such as lipid droplets. Also employed in studies of apoptosis and cellular stress, where changes in fluorescence intensity correlate with membrane integrity and polarity shifts. Its nitro group enhances photostability, allowing prolonged imaging under microscopy. Additionally, it serves as a lead compound in developing sensors for protein-ligand interactions in drug discovery.