Used as a fluorescent probe in biochemical and analytical applications due to its strong emission properties upon binding to specific biomolecules. It is particularly effective in detecting conformational changes in proteins and monitoring enzyme activity. Its nitrobenzothiazole core enhances sensitivity in cellular imaging, making it valuable in research settings for studying intracellular environments. Also employed in the development of sensors for metal ions, where it shows selective response to certain cations through fluorescence quenching or enhancement. Its stability and distinct optical signal support its use in high-throughput screening and diagnostic assays.