Widely used in cell biology research as a fluorescent lipid probe to study membrane dynamics, intracellular trafficking, and lipid metabolism. Its bright fluorescence and photostability allow real-time visualization of lipid behavior in live and fixed cells. Commonly employed to label and track lipid rafts, endocytic pathways, and Golgi apparatus due to its affinity for ordered membrane domains. Also utilized in fluorescence microscopy, flow cytometry, and lipid uptake assays. The long hydrophobic linker ensures proper membrane integration, making it ideal for tracing sphingolipid processing and sphingomyelin distribution in cellular systems.