ML-10 is primarily used in research settings as a fluorescent probe for detecting hypoxia (low oxygen levels) in living cells and tissues. It selectively accumulates in hypoxic environments, making it a valuable tool for studying tumor biology, as many solid tumors exhibit hypoxic regions that are linked to resistance to therapy and poor prognosis. Researchers use ML-10 in imaging applications, particularly in fluorescence microscopy and in vivo imaging, to visualize and quantify hypoxia in real time. Its rapid cellular uptake and quick clearance from normoxic (normal oxygen) areas enhance contrast and improve detection sensitivity. This compound supports the development and monitoring of hypoxia-targeted treatments and contributes to a better understanding of disease mechanisms in cancer and ischemic conditions.