Used as a fluorescent tracer in biological and cellular research, particularly for studying cell membrane permeability and intercellular communication. Its high water solubility and strong fluorescence make it ideal for microinjection studies, where it helps visualize cell morphology and track dye coupling between adjacent cells. Commonly employed in neuroscience to map neuronal connections and in developmental biology to trace cell lineages. The compound is also utilized in fluorescence microscopy due to its photostability and bright yellow-green emission under UV light. It exhibits low toxicity, enabling prolonged use in live cells without causing damage.