Used primarily as a fluorescent probe in biochemical and cellular research due to its strong fluorescence properties. It selectively binds to nucleic acids, making it useful in DNA and RNA staining for microscopy and flow cytometry applications. The compound is also employed in studying cell permeability and intracellular localization because of its ability to cross cell membranes and accumulate in specific organelles. Its amino and dimethylamino functional groups enhance solubility and interaction with biological molecules, supporting its use in live-cell imaging with minimal toxicity. Additionally, it serves as a building block in synthesizing more complex fluorescent tags and sensors for detecting biomolecular interactions.