Used as a fluorescent probe in biological research to study membrane dynamics and lipid rafts due to its environment-sensitive emission properties. It selectively partitions into lipid bilayers and exhibits enhanced fluorescence in hydrophobic environments, making it useful for monitoring changes in membrane fluidity and phase transitions. Also employed in cellular imaging to track endocytosis and intracellular trafficking pathways. Its chloride salt form improves solubility and stability in aqueous buffers, facilitating use in live-cell assays.