Used as a fluorescent probe in biochemical assays due to its strong emission properties in the visible range. Its rigid macrocyclic structure allows selective binding to specific biomolecules, making it suitable for detecting protein–ligand interactions and nucleic acid structures. Commonly employed in cell imaging techniques, especially for tracking membrane dynamics and intracellular pH changes. Also applied in supramolecular chemistry as a building block for sensors and molecular recognition systems. Shows potential in photodynamic therapy owing to its ability to generate singlet oxygen under light irradiation.