EC-23 is widely used in the development of fluorescent probes for biological imaging due to its strong emission in the near-infrared range, allowing deep tissue penetration with minimal background interference. It is particularly effective in real-time monitoring of cellular processes and tracking specific biomolecules in live cells. Its high photostability and low cytotoxicity make it suitable for long-term imaging studies. Additionally, EC-23 has been employed in biosensor platforms for detecting metal ions and small biomolecules, offering high sensitivity and selectivity. The compound also shows promise in photodynamic therapy, where it acts as a photosensitizer to generate reactive oxygen species upon light irradiation, enabling targeted destruction of cancer cells.