Used primarily as a fluorescent probe in biochemical and cellular imaging, this compound binds selectively to specific enzyme active sites, making it valuable for studying metabolic activity in live cells. Its strong fluorescence and photostability allow for prolonged observation under microscopy, especially in mitochondrial function assays. It is also employed in high-throughput screening platforms to monitor enzyme inhibition or activation, particularly in cancer and neurodegenerative disease research. Due to its structural similarity to cofactors like flavins, it can act as a redox sensor, providing real-time insights into cellular oxidation states.