Used as a fluorescent probe in biochemical assays due to its strong electron-withdrawing nitro groups and conjugated structure, enabling detection of reactive oxygen species in cellular environments. Its extended π-conjugation allows for visible-range absorption and emission, making it suitable for imaging applications without requiring UV light. Also investigated as a photosensitizer in photodynamic therapy research, where it generates singlet oxygen upon light activation. Additionally, serves as an intermediate in synthesizing more complex heterocyclic compounds with potential pharmacological activity.