Used as a fluorescent probe in biological imaging due to its strong near-infrared emission and high photostability. It selectively accumulates in mitochondria of living cells, making it valuable for tracking mitochondrial dynamics and membrane potential changes. Commonly applied in confocal microscopy and flow cytometry for real-time monitoring of cellular processes. Also employed in studies of apoptosis and oxidative stress because of its sensitivity to changes in the cellular environment. Its lipophilic cationic structure allows easy penetration through cell membranes, enhancing its utility in live-cell imaging without requiring transfection agents.