Used as a sensitive fluorescent probe in biological imaging due to its ability to penetrate cell membranes and emit strong fluorescence upon interaction with cellular components. Commonly employed in assays to detect mitochondrial activity and monitor membrane potential changes in live cells. Also utilized in flow cytometry and fluorescence microscopy for cell viability studies. Its triphenylmethyl group enhances lipophilicity, improving cellular uptake and localization in hydrophobic environments such as lipid bilayers.