This chemical is widely used in bioconjugation and labeling applications due to its dual PEGylated structure and Cy5 fluorescent dye. The PEG chains enhance solubility and reduce immunogenicity, making it suitable for in vivo studies. The Cy5 dye allows for deep-red fluorescence imaging, which is ideal for tracking biomolecules in complex biological systems. It is commonly employed in fluorescence microscopy, flow cytometry, and in vivo imaging to study cellular processes, protein interactions, and drug delivery mechanisms. The presence of an acid group enables easy covalent attachment to amines or other reactive groups on target molecules, facilitating the creation of stable conjugates for research and diagnostic purposes.