Used as a fluorescent labeling reagent in biochemical and immunological assays. Its primary application is in the detection and quantification of biomolecules such as proteins and antibodies due to its ability to form stable conjugates with amine groups. The compound is particularly useful in enzyme-linked immunosorbent assays (ELISA) and other immunoassay techniques where high sensitivity is required. It enhances detection limits by providing strong fluorescent signals upon excitation, allowing for precise measurement of low-abundance targets. Also employed in cellular imaging to track molecular interactions in live or fixed cells, leveraging its cell permeability and low background fluorescence.