Used as a fluorescent probe to study cellular processes, particularly in tracking and visualizing amine groups in proteins and peptides. Its fluorescence properties make it valuable in assays detecting enzymatic activity, especially transglutaminase, which plays a role in cell death and tissue remodeling. Commonly applied in microscopy and flow cytometry to label and monitor intracellular components due to its ability to penetrate cell membranes. Also utilized in research on autophagy and apoptosis, helping distinguish between different cell death pathways.