Used in bioconjugation for labeling biomolecules such as antibodies, peptides, and nucleic acids, enabling fluorescence detection in imaging and diagnostic applications. Commonly applied in flow cytometry, fluorescence microscopy, and real-time PCR for high sensitivity detection due to its strong absorbance and emission in the near-infrared range. Facilitates site-specific labeling when incorporated into oligonucleotides or proteins during synthesis. Also employed in FRET assays as a donor or acceptor fluorophore to study molecular interactions. Stable under physiological conditions, making it suitable for live-cell imaging and in vivo studies.