Lucifer Yellow CH dipotassium salt is widely used in biological research as a fluorescent dye for cell labeling and tracing. Its high fluorescence intensity and stability make it ideal for studying cell-to-cell communication, particularly in neuroscience, where it is used to trace neuronal pathways and map synaptic connections. The dye is often injected into cells to visualize their morphology and track the spread of molecules through gap junctions. Additionally, it is employed in studies involving endocytosis and membrane dynamics due to its ability to remain within cells without significant leakage. Its compatibility with living cells and tissues makes it a valuable tool for real-time imaging and long-term experiments.