This chemical is primarily used in the field of molecular biology and biochemistry as a fluorescent probe for detecting reactive oxygen species (ROS) in cellular environments. Its application is crucial in studying oxidative stress, a condition where excessive ROS production damages cellular components like DNA, proteins, and lipids. Researchers utilize it to monitor ROS levels in live cells, tissues, or biological samples, providing insights into cellular health, disease mechanisms, and the efficacy of antioxidants. Additionally, it is employed in high-throughput screening assays to identify compounds that modulate oxidative stress, making it valuable in drug discovery and development. Its fluorescence properties allow for real-time imaging and quantification, enhancing the understanding of ROS-related processes in various biological systems.