Used extensively in biological research for DNA staining and fluorescence microscopy. It binds selectively to the minor groove of double-stranded DNA, making it valuable for visualizing chromosomes and nuclear structures in cells. Its application extends to flow cytometry, where it helps in analyzing cell cycle stages and DNA content. Additionally, it is employed in studies involving apoptosis and cell viability assays due to its ability to differentiate between live and dead cells based on DNA integrity. Its fluorescent properties also make it useful in high-throughput screening and drug discovery processes.