Used extensively in biological research for staining DNA in cells, particularly in fluorescence microscopy and flow cytometry. It binds selectively to the minor groove of double-stranded DNA, making it valuable for visualizing nuclear material and studying cell cycle dynamics. Its application extends to apoptosis detection, where it helps identify fragmented DNA in dying cells. Additionally, it is employed in chromatin studies to analyze DNA-protein interactions and in drug discovery to screen compounds that affect DNA structure. Its fluorescence properties also make it useful in quantitative DNA assays and high-throughput screening platforms.