EMA is widely used in molecular biology for selective DNA analysis in samples containing both live and dead cells. It binds to DNA in dead cells, preventing their amplification in PCR, thus allowing accurate detection of viable microorganisms. This property is particularly useful in environmental monitoring, food safety testing, and clinical diagnostics to assess microbial viability. EMA is also employed in fluorescence microscopy to differentiate between live and dead cells based on DNA staining. Its application ensures more reliable results in studies focused on cell viability and pathogen detection.