DiBAC4(5) is widely used as a fluorescent dye for monitoring membrane potential in living cells. It is particularly valuable in electrophysiology and cell biology studies, where it helps researchers assess changes in cellular membrane potential in real-time. The dye enters depolarized cells and exhibits increased fluorescence, making it a useful tool for studying ion channel activity, drug effects on membrane potential, and cellular responses to various stimuli. Its application extends to neuroscience, cardiology, and pharmacology, aiding in the understanding of cellular excitability and function. DiBAC4(5) is also employed in high-throughput screening assays to identify compounds that modulate membrane potential, contributing to drug discovery and development.