Filipin III is widely used in biological research as a fluorescent probe to detect and visualize cholesterol in cell membranes. Its ability to bind specifically to cholesterol makes it a valuable tool for studying the distribution and dynamics of cholesterol within cellular structures. Researchers utilize Filipin III in fluorescence microscopy to observe cholesterol-rich domains, such as lipid rafts, and to investigate the role of cholesterol in various cellular processes, including membrane organization and signaling. Additionally, it is employed in studies related to cholesterol-related diseases, such as Niemann-Pick type C, to assess cholesterol accumulation in cells. Its application provides critical insights into the biological functions and pathological implications of cholesterol in cellular systems.