This chemical is widely used as a fluorescent probe in biological and biochemical research. It is particularly valuable in detecting reactive oxygen species (ROS) within cells, helping researchers study oxidative stress and its impact on cellular processes. Its high sensitivity and specificity make it a preferred choice for assays involving live-cell imaging and flow cytometry. Additionally, it is employed in environmental monitoring to assess oxidative damage in ecosystems caused by pollutants. Its stable fluorescence under various conditions ensures reliable results in both laboratory and field studies.