Resorufin pentyl ether is primarily used in biochemical research as a fluorescent probe. It is particularly valuable in assays that measure enzyme activity, especially those involving oxidoreductases. The compound's fluorescence properties make it suitable for detecting and quantifying reactive oxygen species (ROS) in cellular environments. It is also employed in studying oxidative stress and antioxidant mechanisms within biological systems. Additionally, Resorufin pentyl ether is utilized in high-throughput screening (HTS) to identify potential drug candidates that modulate enzymatic pathways. Its stability and sensitivity in various pH conditions further enhance its applicability in diverse experimental setups.