TMA-DPH is widely used as a fluorescent probe in biological and biophysical research. Its primary application is in studying cell membrane properties, particularly membrane fluidity and lipid organization. Due to its amphiphilic nature, it readily incorporates into lipid bilayers, making it a valuable tool for monitoring changes in membrane dynamics under various conditions, such as temperature variations or the presence of membrane-active compounds. Additionally, TMA-DPH is employed in fluorescence anisotropy measurements to assess the rotational mobility of molecules within membranes, providing insights into membrane microenvironments. It is also utilized in studies involving membrane potential and the interaction of membranes with proteins or drugs, contributing to a deeper understanding of cellular processes and drug delivery mechanisms.