Lucifer Yellow CH dilithium salt is widely used as a fluorescent dye in biological and neurobiological research. Its primary application is in tracing and labeling cells, particularly neurons, due to its ability to pass through gap junctions. This property makes it valuable for studying cell-to-cell communication and neuronal networks. It is also employed in fluorescence microscopy to visualize cellular structures and dynamics. Additionally, it is used in intracellular injection studies to track the movement and morphology of cells over time. Its high fluorescence intensity and stability under various conditions make it a reliable tool for long-term imaging experiments.