DAF-2 is widely used as a fluorescent probe for the detection and quantification of nitric oxide (NO) in biological systems. When DAF-2 reacts with NO in the presence of oxygen, it forms a highly fluorescent triazole compound, DAF-2T, which allows for the visualization and measurement of NO levels in cells and tissues. This application is particularly valuable in research focused on understanding the role of nitric oxide in various physiological and pathological processes, such as vascular function, immune response, and neurotransmission. DAF-2 is commonly employed in cell culture studies, tissue imaging, and in vivo experiments to monitor NO dynamics in real-time, providing insights into its signaling mechanisms and biological impact.